Journal: Journal of Experimental & Clinical Cancer Research : CR
Article Title: Progesterone boosts abiraterone-driven target and NK cell therapies against glioblastoma
doi: 10.1186/s13046-024-03144-2
Figure Lengend Snippet: Effect of Prog on MGMT expression. A , B After treatment with Prog in the absence and presence of Abi, T98G cells were harvested for Western blotting (Left) and RT-qPCR (Right). C Left: After treatment for 48 h, T98G cells were treated with 20 µM cycloheximide (CHX) for the indicated time interval, and cell lysates were collected for Western blotting. Right: Quantitative results. D After treatment with Prog, gDNA of T98G and U87MG-R cells F was purified, and subjected to pyrosequencing analysis. The percentage of methylation in 5 CpG residues in T98G and U87MG-R cells were quantified. Experiments were performed independently three times. (* P < 0.05, ** P < 0.01)
Article Snippet: CpGs methylation are tested with the PyroMark Q24 CpG MGMT kit provided by Qiagen [ , ].
Techniques: Expressing, Western Blot, Quantitative RT-PCR, Purification, Methylation